Proteomic Dynamics and Heat Stress Response inArabidopsisSeedlings

Abstract

AbstractGlobal warming poses a grave threat to plant survival, adversely affecting growth and agricultural productivity. To develop thermotolerant crops, a profound comprehension of plant responses to heat stress at the molecular level is imperative. Leveraging a novel fusion of 15N-stable isotope labeling and the ProteinTurnover algorithm, we meticulously investigated proteome dynamics in Arabidopsis thaliana seedlings subjected to moderate heat stress (30°C). This innovative approach facilitated a comprehensive analysis of proteomic changes across diverse cellular fractions. Our study unveiled significant turnover rate alterations in 571 proteins, with a median increase of 1.4-fold, indicative of accelerated protein dynamics under heat stress. Notably, root soluble proteins exhibited more subdued changes, suggesting tissue-specific adaptations. Moreover, we observed noteworthy turnover variations in proteins associated with redox signaling, stress response, and metabolism, underscoring the complexity of the response network. Conversely, proteins involved in carbohydrate metabolism and mitochondrial ATP synthesis displayed minimal turnover changes, signifying their stability. This exhaustive examination sheds light on the proteomic adjustments of Arabidopsis seedlings to moderate heat stress, elucidating the delicate balance between proteome stability and adaptability. These findings significantly augment our understanding of plant thermal resilience and offer crucial insights for the development of crops endowed with enhanced thermotolerance.