GIBBERELLIN SIGNALING THROUGH RGA SUPPRESSES GCN5 EFFECT ON STAMEN ELONGATION OF ARABIDOPSIS FLOWERS

Abstract

AbstractHistone acetyltransferases (HAT) modify the amino-terminal tails of the core histone proteins via acetylation, regulating chromatin structure and transcription. The GENERAL CONTROL NON-DEREPRESSIBLE 5 (GCN5) is a HAT that specifically acetylates H3K14 residues. GCN5 has been associated with cell division and differentiation, meristem function, root, stem, foliar and floral development, and plant environmental response. The flowers ofgcn5–6plants display reduced length of stamen and exhibit male sterility relative to the wild-type plants. We show these effects may arise from gibberellin (GA) signaling defects. The signaling pathway of bioactive GAs depends on the proteolysis of their repressors, DELLA proteins. The DELLA protein, REPRESSOR OF GA (RGA), represses plant growth, inflorescence, flower and seed development. Our molecular data indicate that GCN5 is required for activation and H3K14 acetylation of genes involved in the late stages of GA biosynthesis and catabolism. We studied the genetic interaction of RGA and GCN5; RGA can partially suppress GCN5 action. The reduced elongation of the stamen filament ofgcn5–6mutants is reversed in therga–t2;gcn5–6double mutants. This mechanism involved suppressing the GCN5 effect on the expression and histone acetylation inGAI-locus by RGA. Interestingly, RGA and RGL2 do not suppress ADA2b function, suggesting that ADA2b acts downstream in GA signaling and is distinct from GCN5 activity. In conclusion, we propose that the action of GCN5 on stamen elongation is mediated partially by RGA and GA signaling.