Abstract
AbstractSubstantial efforts are underway that aim to deepen our understanding of human brain morphology, structure and function using high-resolution imaging as well has high-content molecular profiling technologies. The current work adds to these efforts by providing a comprehensive and quantitative protein expression map of 13 anatomically distinct brain regions covering more than 10,000 proteins. This was enabled by the optimization, characterization and implementation of a high-sensitivity and high-throughput micro-flow liquid chromatography timsTOF tandem mass spectrometry system (LC-MS/MS) capable of analyzing >2,000 consecutive samples prepared from formalin fixed paraffin embedded (FFPE) material. Analysis of this proteomic resource highlighted e.g. brain region-enriched protein expression patterns and functional protein classes, protein localization differences between brain regions and individual protein markers for specific brain regions. To facilitate access to and ease further mining of the data by the scientific community, all data can be explored online in a purpose-built Shiny App (https://brain-region-atlas.proteomics.ls.tum.de).
Publisher
Cold Spring Harbor Laboratory
Reference41 articles.
1. The PsychENCODE project
2. The Human Brain Project—Synergy between neuroscience, computing, informatics, and brain-inspired technologies
3. Equal numbers of neuronal and nonneuronal cells make the human brain an isometrically scaled-up primate brain
4. Proteome profiling in cerebrospinal fluid reveals novel biomarkers of Alzheimer’s disease;Mol Syst Biol,2020
5. An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes;Bekker-Jensen DB, Kelstrup CD, Batth TS, Larsen SC, Haldrup C, Bramsen JB, Sørensen KD, Høyer S, Ørntoft TF, Andersen CL;Cell Syst,2017
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