Opposite polarity programs regulate asymmetric subsidiary cell divisions in grasses

Abstract

Stomata are cellular pores on the leaf epidermis that Grass stomata recruit lateral subsidiary cells (SCs), which are key to the unique stomatal morphology and the efficient plant-atmosphere gas exchange in grasses. Subsidiary mother cells (SMCs) strongly polarise before an asymmetric division forms a SC. Yet apart from a proximal polarity module that includes PANGLOSS1 (PAN1) and guides nuclear migration, little is known regarding the developmental processes that form SCs. Using the genetic model grass Brachypodium distachyon, we identified BdPOLAR, which forms a novel, distal polarity domain in SMCs that is reciprocal to the proximal PAN1 domain. Both polarity domains are required for the formative SC division yet exhibit distinct roles in regulating pre-mitotic nuclear migration and SMC division plane orientation, respectively. Nonetheless, the domains are linked as the proximal domain controls polarisation of the distal domain. In summary, we identified two opposing polarity domains that coordinate the SC division, a process crucial for grass stomatal physiology.