Cdc73 majorly regulates apoptosis-inducing factor (AIF1) inSaccharomyces cerevisiaevia the H3K36 methylation

Abstract

AbstractAIF1overexpression is intimately linked to the sensitivity of the yeast cells towards hydrogen peroxide or acetic acid. Therefore, studying the mechanism of its regulation in the cell would provide a significant understanding of the factors ultimately guiding yeast apoptosis. In this report, we establish the time-dependent induction ofAIF1in hydrogen peroxide stress. Additionally, theAIF1expression in hydrogen peroxide is mediated by two transcription factors, Yap5 (DNA binding) and Cdc73 (non-DNA binding). Furthermore, substituting the H3K36 residue with another significantly abrogates theAIF1expression. However, substituting H3K4 or H3K79 with A does not affect theAIF1expression level under hydrogen peroxide stress. Altogether, the significant reduction ofAIF1expression incdc73Δ cells plausibly reflects the reduced H3K36me3 modification and is independent of the H3K4me3 modification.