TheSilene latifoliagenome and its giant Y chromosome
Author:
Moraga Carol, Branco Catarina, Rougemont Quentin, Veltsos ParisORCID, Jedlička PavelORCID, Muyle Aline, Hanique Melissa, Tannier Eric, Liu XiaodongORCID, Mendoza-Galindo EddyORCID, Lemaitre ClaireORCID, Fields Peter D.ORCID, Cruaud Corinne, Labadie Karine, Belser CarolineORCID, Briolay Jerome, Santoni Sylvain, Cegan RadimORCID, Linheiro Raquel, de la Vega Ricardo C. RodríguezORCID, Adam Gabriele, Filali Adil ElORCID, Mossion VincianeORCID, Boualem AdnaneORCID, Tavares Raquel, Chebbi Amine, Cordaux Richard, Fruchard Cécile, Prentout DjivanORCID, Velt Amandine, Spataro Bruno, Delmotte Stephane, Weingartner LauraORCID, Toegelová Helena, Tulpová Zuzana, Cápal Petr, Šimková HanaORCID, Štorchová HelenaORCID, Krüger Manuela, Abeyawardana Oushadee A. J., Taylor Douglas R., Olson Matthew S.ORCID, Sloan Daniel B.ORCID, Karrenberg SophieORCID, Delph Lynda F.ORCID, Charlesworth DeborahORCID, Giraud TatianaORCID, Bendahmane AbdelhafidORCID, Genova Alex DiORCID, Madoui AminORCID, Hobza Roman, Marais Gabriel A. B.ORCID
Abstract
AbstractIn some species, the Y is a tiny chromosome but the dioecious plantSilene latifoliahas a giant ∼550 Mb Y chromosome, which has remained unsequenced so far. Here we used a hybrid approach to obtain a high-quality maleS. latifoliagenome. Using mutants for sexual phenotype, we identified candidate sex-determining genes on the Y. Comparative analysis of the sex chromosomes with outgroups showed the Y is surprisingly rearranged and degenerated for a ∼11 MY-old system. Recombination suppression between X and Y extended in a stepwise process, and triggered a massive accumulation of repeats on the Y, as well as in the non-recombining pericentromeric region of the X, leading to giant sex chromosomes.One-Sentence SummaryThis work uncovers the structure, function, and evolution of one of the largest giant Y chromosomes, that of the model plantSilene latifolia, which is almost 10 times larger than the human Y, despite similar genome sizes.
Publisher
Cold Spring Harbor Laboratory
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