Abstract
AbstractFlagellotropic bacteriophages are interesting candidates as therapeutics against pathogenic bacteria dependent on flagellar motility for colonization and causing disease. Yet, phage resistance other than loss of motility has been scarcely studied. Here we developed a soft agar assay to study flagellotropic phage F341 resistance in motileCampylobacter jejuni. We found that phage adsorption was prevented by diverse genetic mutations in the lipooligosaccharides forming the secondary receptor of phage F341. Genome sequencing showed phage F341 belongs to theFletchervirusgenus otherwise comprising capsular-dependentC. jejuniphages. Interestingly, phage F341 encodes a hybrid receptor binding protein (RBP) predicted as a short tail fiber showing partial similarity to RBP1 encoded by capsular-dependentFletchervirus,but with a receptor binding domain similar to tail fiber protein H ofC. jejuniCJIE1 prophages. Thus,C. jejuniprophages may represent a genetic pool from where lyticFletchervirusphages can acquire new traits like recognition of new receptors.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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