An enhanced isothermal amplification assay for viral detection

Author:

Qian JasonORCID,Boswell Sarah A.ORCID,Chidley ChristopherORCID,Lu Zhi-xiangORCID,Pettit Mary E.ORCID,Gaudio Benjamin L.ORCID,Fajnzylber Jesse M.,Ingram Ryan T.,Ward Rebecca H.,Li Jonathan Z.,Springer MichaelORCID

Abstract

AbstractRapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. We developed a molecular diagnostic test for SARS-CoV-2, FIND (Fast Isothermal Nucleic acid Detection), based on an enhanced isothermal recombinase polymerase amplification reaction. FIND has a detection limit on patient samples close to that of RT-qPCR, requires minimal instrumentation, and is highly scalable and cheap. It can be performed in high throughput, does not cross-react with other common coronaviruses, avoids bottlenecks caused by the current worldwide shortage of RNA isolation kits, and takes ~45 minutes from sample collection to results. FIND can be adapted to future novel viruses in days once sequence is available.One sentence summarySensitive, specific, rapid, scalable, enhanced isothermal amplification method for detecting SARS-CoV-2 from patient samples.

Publisher

Cold Spring Harbor Laboratory

Reference38 articles.

1. John Hopkins Coronavirus Resource Center. coronavirus.jhu.edumap.html.

2. A. Park , Dr. Anthony Fauci “Not Overly Confident” With U.S. COVID-19 Testing Capabilities. time.comanthony-fauci-covid--testing-capabilities.

3. J. Temple , Why contact tracing may be a mess in America.

4. Emergency use authorization granted for new biomaterial collection approach. www.rutgers.edunewsnew-rutgers-saliva-test-coronavirus-gets-fda-approval.

5. S. Srivatsan et al., Preliminary support for a “dry swab, extraction free” protocol for SARS-CoV-2 testing via RT-qPCR. bioRxiv, 2020.04.22.056283 (2020).

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