Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
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Published:2020-08-26
Issue:12
Volume:4
Page:1140-1149
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ISSN:2157-846X
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Container-title:Nature Biomedical Engineering
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language:en
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Short-container-title:Nat Biomed Eng
Author:
Patchsung Maturada, Jantarug Krittapas, Pattama Archiraya, Aphicho Kanokpol, Suraritdechachai Surased, Meesawat Piyachat, Sappakhaw Khomkrit, Leelahakorn Nattawat, Ruenkam Theerawat, Wongsatit Thanakrit, Athipanyasilp Niracha, Eiamthong Bhumrapee, Lakkanasirorat Benya, Phoodokmai Thitima, Niljianskul Nootaree, Pakotiprapha DanayaORCID, Chanarat Sittinan, Homchan AimornORCID, Tinikul Ruchanok, Kamutira Philaiwarong, Phiwkaow Kochakorn, Soithongcharoen Sahachat, Kantiwiriyawanitch Chadaporn, Pongsupasa Vinutsada, Trisrivirat Duangthip, Jaroensuk Juthamas, Wongnate Thanyaporn, Maenpuen Somchart, Chaiyen Pimchai, Kamnerdnakta Sirichai, Swangsri Jirawat, Chuthapisith Suebwong, Sirivatanauksorn Yongyut, Chaimayo Chutikarn, Sutthent Ruengpung, Kantakamalakul Wannee, Joung JuliaORCID, Ladha Alim, Jin Xin, Gootenberg Jonathan S.ORCID, Abudayyeh Omar O., Zhang Feng, Horthongkham NavinORCID, Uttamapinant ChayasithORCID
Funder
The project is financially supported by PTT, PTTEP, Kasikorn Bank, Siam Commercial Bank, GPSC, VISTEC, and Siriraj Hospital.
Publisher
Springer Science and Business Media LLC
Subject
Computer Science Applications,Biomedical Engineering,Medicine (miscellaneous),Bioengineering,Biotechnology
Reference31 articles.
1. Vogels, C. B. F. et al. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets. Nat. Microbiol. https://doi.org/10.1038/s41564-020-0761-6 (2020). 2. Kelly-Cirino, C. D. et al. Importance of diagnostics in epidemic and pandemic preparedness. BMJ Glob. Health 4, e001179 (2019). 3. Piepenburg, O., Williams, C. H., Stemple, D. L. & Armes, N. A. DNA detection using recombination proteins. PLoS Biol. 4, e204 (2006). 4. Notomi, T. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, e63 (2000). 5. Rabe, B. A. & Cepko, C. SARS-CoV-2 detection using an isothermal amplification reaction and a rapid, inexpensive protocol for sample inactivation and purification. Preprint at https://doi.org/10.1101/2020.04.23.20076877 (2020).
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