Author:
Reuß Daniel R.,Altenbuchner Josef,Mäder Ulrike,Rath Hermann,Ischebeck Till,Sappa Praveen Kumar,Thürmer Andrea,Guérin Cyprien,Nicolas Pierre,Steil Leif,Zhu Bingyao,Feussner Ivo,Klumpp Stefan,Daniel Rolf,Commichau Fabian M.,Völker Uwe,Stülke Jörg
Abstract
Understanding cellular life requires a comprehensive knowledge of the essential cellular functions, the components involved, and their interactions. Minimized genomes are an important tool to gain this knowledge. We have constructed strains of the model bacterium, Bacillus subtilis, whose genomes have been reduced by ∼36%. These strains are fully viable, and their growth rates in complex medium are comparable to those of wild type strains. An in-depth multi-omics analysis of the genome reduced strains revealed how the deletions affect the transcription regulatory network of the cell, translation resource allocation, and metabolism. A comparison of gene counts and resource allocation demonstrates drastic differences in the two parameters, with 50% of the genes using as little as 10% of translation capacity, whereas the 6% essential genes require 57% of the translation resources. Taken together, the results are a valuable resource on gene dispensability in B. subtilis, and they suggest the roads to further genome reduction to approach the final aim of a minimal cell in which all functions are understood.
Funder
GGNB
Deutsche Forschungsgemeinschaft
Publisher
Cold Spring Harbor Laboratory
Subject
Genetics (clinical),Genetics
Cited by
128 articles.
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