Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient
Author:
Harcourt Jennifer, Tamin Azaibi, Lu Xiaoyan, Kamili Shifaq, Sakthivel Senthil Kumar., Murray Janna, Queen Krista, Tao Ying, Paden Clinton R.ORCID, Zhang Jing, Li Yan, Uehara Anna, Wang Haibin, Goldsmith Cynthia, Bullock Hannah A., Wang Lijuan, Whitaker Brett, Lynch Brian, Gautam Rashi, Schindewolf Craig, Lokugamage Kumari G., Scharton Dionna, Plante Jessica A.ORCID, Mirchandani Divya, Widen Steven G., Narayanan Krishna, Makino Shinji, Ksiazek Thomas G., Plante Kenneth S., Weaver Scott C., Lindstrom Stephen, Tong Suxiang, Menachery Vineet D.ORCID, Thornburg Natalie J.ORCID
Abstract
ABSTRACTThe etiologic agent of the outbreak of pneumonia in Wuhan China was identified as severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) in January, 2020. The first US patient was diagnosed by the State of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens, and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into two virus repositories, making it broadly available to the public health and research communities. We hope that open access to this important reagent will expedite development of medical countermeasures.Article SummaryScientists have isolated virus from the first US COVID-19 patient. The isolation and reagents described here will serve as the US reference strain used in research, drug discovery and vaccine testing.
Publisher
Cold Spring Harbor Laboratory
Reference25 articles.
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