Analysis of polyurethane/gelatin complex hydrogel system for protein imprinting

Author:

Porchkhidze Alegi,Endeladze Madona,Gogichaishvili Nana,Mikautadze Otar

Abstract

AbstractPolyurethane served as the carrier in the synthesis of a hydrogel system, incorporating membrane protein as the template, N-Vinylformamide as the monomer, and 1,4-Butanediol diglycidyl ether as the crosslinker, along with gelatin, initiated by ultraviolet radiation. This resulted in the formation of the hydrogel PUNVF-Gelatin. The study investigated the influence of varying monomer concentration, crosslinker concentration, and gelatin concentration on both the adsorption capacity for membrane protein and the imprinting efficiency. Findings revealed that optimal conditions for achieving the maximum adsorption capacity occurred when the monomer mass fraction was 5%, the crosslinker mass fraction was 3%, and the gelatin mass fraction was 0.6%.

Publisher

Cold Spring Harbor Laboratory

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