Author:
Little Tom J.,O’Toole Aine N.,Rambaut Andrew,Chandra Tamir,Marioni Riccardo,Pedersen Amy B.
Abstract
AbstractThe age structure of populations, or the ageing rate of individuals, impacts aspects of ecology, epidemiology and conservation. Yet for many wild organisms, age is an inaccessible trait. In many cases measuring age or ageing rates in the wild requires molecular biomarkers of age. Epigenetic clocks based on DNA methylation have been shown to accurately estimate the age of humans and laboratory mice, but they also show variable ticking rates that are associated with mortality risk above and beyond that predicted by chronological age. Thus, epigenetic clocks are proving to be useful markers of both chronological and biological age, and they are beginning to be applied to wild mammals and birds. We have acquired strong evidence that an accurate clock is possible for the wood mouseApodemus sylvaticusby adapting epigenetic information from the laboratory mouse (Mus musculus).Apodemus sylvaticus isa well-studied, common small mammal in the UK and Europe, which is amenable to large-scale experimental perturbations and longitudinal sampling of individuals across their lives. These features of the wood mouse system offer opportunities to disentangle causal relationships between ageing rates and environmental stress. Our wood mouse epigenetic clock is PCR-based, and so requires only tiny amounts of tissue accessible through non-destructive sampling. We quantified methylation using Oxford Nanopore sequencing technology and present a new bioinformatics pipeline for data analysis. We thus describe a new and generalizable system that should enable ecologists and other field biologists to go from small tissue samples to an epigenetic clock for their study animal, which will enable investigations of ageing in the wild which where previously inaccessible.
Publisher
Cold Spring Harbor Laboratory
Reference29 articles.
1. Eleven Telomere, Epigenetic Clock, and Biomarker-Composite Quantifications of Biological Aging: Do They Measure the Same Thing?;American Journal of Epidemiology,2017
2. Faster ticking rate of the epigenetic clock is associated with faster pubertal development in girls;Epigenetics,2018
3. DNA methylation-based measures of biological age: Meta-analysis predicting time to death;Aging,2016
4. Parasitic nematodes simultaneously suppress and benefit from coccidian coinfection in their natural mouse host;Parasitology,2019
5. Age estimation in a long-lived seabird (Ardenna tenuirostris) using DNA methylation-based biomarkers;Molecular Ecology Resources,2019
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