Author:
Timohhina Natalja,Cerrato Carmine Pasquale,Kurrikoff Kaido,Chekulayev Vladimir,Aid-Vanakova Jekaterina,Puurand Marju,Tepp Kersti,Shevchuk Igor,Langel Ulo,Kaambre Tuuli
Abstract
AbstractThe popularity of the specially synthesized cell-penetrating peptides (CPPs) in cancer treatment has grown recently. The main aim of this study was to investigate the effects of four mitochondrially targeted antioxidant CPPs on the viability and bioenergetic function of mitochondria in human adenocarcinoma Caco-2 cells. The number of viable cells was measured by MTT and trypan blue assays. Respirometry and the permeabilized cell technique were applied to measure the mitochondrial function in this cell line. We did not observe any significant effect of CPPs on the mitochondrial reserve respiratory capacity, the function of respiratory chain complexes, and the inclination of these cancer cells to aerobic glycolysis. mtgCPP peptide with the highest antioxidant activity demonstrated improved mitochondrial coupling efficiency. CPPs do not affect mitochondrial function directly but can be considered in therapeutics as a drug-delivery molecule
Publisher
Cold Spring Harbor Laboratory