Author:
Liu Xiaoxue,Wu Youzhi,Jiao Lingxia,Ran Junjian,Sun Linjun,Ye Fuzhou,Liang Xinhong,Zhao Ruixiang
Abstract
AbstractAcid-responsive proteome expression profiles ofAlicyclobacillus acidoterrestris(A. acidoterrestris) were analysed using label-free quantitative mass spectrometry to investigate its acid resistance mechanism at sublethal pH. Totally, 325 differential expression proteins were identified during acid stress at pH2.5 condition for 15 min, of which the expressions of 83 proteins were up-regulated and the other 242 proteins expressions were down-regulated. Differentially expressed proteins were mainly involved in organic nitrogen compounds metabolism, small molecule metabolism, organic acid metabolism and signal transduction. Overall, they were mapped into 97 metabolic pathways. Combination of KEGG pathway analysis and protein functional analysis suggested that the pH homeostasis system, changes in metabolic pathways, cell membrane permeability and DNA repair are the main acid resistance mechanisms ofA. acidoterrestrisat sublethal pH conditions. It is speculated thatA. acidoterrestrismay sense and transmit pH signals from the external environment through the nhaB protein which holds histidine-dependent acid resistance system, initiating a series of acid tolerance reactions. Our study demonstrated global physiological response ofA. acidoterrestristo sublethal pH, which provided a better understanding of acid adaption mechanism ofA. acidoterrestris.
Publisher
Cold Spring Harbor Laboratory