Consistent decrease in conifer embolism resistance from the stem apex to base resulting from axial trends in tracheid and pit traits

Abstract

AbstractDrought-induced embolism formation in conifers is associated with several tracheid and pit traits, which vary in parallel from stem apex to base. We tested whether this axial anatomical variability is associated with a progressive variation in embolism vulnerability along the stem from apex to base.We assessed the xylem pressure at 50% loss of conductivity (P50), the tracheid hydraulic diameter (Dh) and mean pit membrane area (PMA) on longitudinal stem segments extracted at different distances from the stem apex (DFA) in aPicea abiesand anAbies albatree. In both trees,DhandPMAscaled withDFA0.2.P50varied for more than 3 MPa from the treetop to the stem base, according to a scaling of -P50withDFA-0.2. The largestDh,PMAandP50variation occurred forDFA<1.5 m.PMAandDhscaled isometrically (exponentb=1).Pit traits vary proportionally with tracheid lumen diameter. Apex-to-base trends in tracheid and pit traits determine a largeDFA-dependentP50variability. Such aDFAeffect onP50did not receive sufficient attention so far, although analysing the relationshipsP50vs.DFAis fundamental for the assessment of embolism vulnerability at the individual level.HighlightsConifer embolism vulnerability depends on pit properties, in agreement with published data.Pit dimensions increase with tracheid lumen diameter, in agreement with published dataTracheid lumen diameter and pit dimensions increase progressively from the stem apex to base, in agreement with published data.Xylem vulnerability to embolism formation (P50) varies for > 3 MPa from the stem apex to base, with the largest variation occurring within 1.5 m from the stem apex.Axial anatomical patterns should be accounted for when analyzing hydraulic properties at individual, intra- and inter-specific scales.