Repression ofMRP51in cis does not contribute to the synthetic growth defect conferred by anhphMX4-marked deletion ofDBP1in aded1-tsmutant

Abstract

ABSTRACTPowers et al. recently demonstrated that thehphMX6cassette used to deleteDPB1indbp1Δ::hphMX6yeast mutants leads to reduced expression incisof the adjacent geneMRP51, encoding the mitochondrial small subunit (SSU) ribosomal protein Mrp51. Here we provide evidence that elimination of Dbp1, not reducedMRP51expression, underlies the synthetic growth defect of adbp1Δ::hphMX6 ded1-tsmutant on glucose-containing medium, where respiration is dispensable, consistent with our previous conclusion that Dbp1 and Ded1 perform overlapping functions in stimulating translation initiation on mRNAs burdened with long or structured 5’UTRs in cells cultured with glucose.