k2v: A Containerized Workflow for Creating VCF Files from Kintelligence Targeted Sequencing Data

Abstract

AbstractThe ForenSeq Kintelligence kit developed by Verogen is a targeted Illumina sequencing assay that genotypes 10,230 single nucleotide polymorphisms designed for forensic genetic genealogy, forensic DNA phenotyping, and ancestry inference. We developedk2v, a containerized workflow for creating standard specification-compliant variant call format (VCF) files from the custom output data produced by the Kintelligence Universal Analysis Software. VCF files produced withk2venable the use of many pre-existing, widely used, community-developed tools for manipulating and analyzing genetic data in the standard VCF format. Here we describe thek2vimplementation, demonstrate its usage, and use the VCF produced byk2vto demonstrate downstream analyses that can easily be performed with pre-existing tools using VCF data as input: concordance analysis, ancestry inference, and relationship estimation.k2vis distributed as a Docker container available on Docker Hub. Documentation and source code fork2vis freely available under the GNU Public License (GPL-3.0) athttps://github.com/signaturescience/k2v.