Author:
Rinkenberger Julie L.,Horning Susan,Klocke Barbara,Roth Kevin,Korsmeyer Stanley J.
Abstract
We disrupted the Mcl-1 locus in murine ES cells to determine the developmental roles of this Bcl-2 family member. Deletion of Mcl-1 resulted in peri-implantation embryonic lethality. Mcl-1−/− embryos do not implant in utero, but could be recovered at E3.5–4.0. Null blastocysts failed to hatch or attach in vitro, indicating a trophectoderm defect, although the inner cell mass could grow in culture. Of note, Mcl-1−/−blastocysts showed no evidence of increased apoptosis, but exhibited a delay in maturation beyond the precompaction stage. This model indicates that Mcl-1 is essential for preimplantation development and implantation, and suggests that it has a function beyond regulating apoptosis.
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
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