Abstract
AbstractStaphylococcus aureusis a pathogen that has developed resistance to each new antibiotic introduced for half a century especially through the acquisition of themecAgene. This bacterial resistance to antibiotics represents a major public health problem. New revolutions are underway, in particular the design of drugs and vaccines targeting the system for detecting the regulatory quorum of the accessory gene (agr). It has been shown that the pathogenicity and resistance ofS. aureuscan be modulated through the intervention of this system. For this reason, we propose in this present work, a new therapeutic design based on anin silicostudy to identify plant miRNAs that could target this system as well as themecAgene based on recent studies showing the inter-realm regulation of human transcripts by plant miRNAs. Out of a total of 20643 miRNAs from mature plants, we identified 29 miRNAs, obtained by the selection criteria MFE ≥ -25 kcal / mol, which could potentially target selected genes ofS. aureus. Fifteen of them were selected on the basis of their thermodynamic stability. Interestingly, The seeds of ptc - miR171g and ptc - miR171h (Populus trichocarpa) both of which almost similar mature miRNA sequence was found to target UCCC region ofRNAIIIgene. miRNAs from plants targetingS. aureushave the potential to be developed as an alternative therapy of the future. To our knowledge, it is the first therapeutic alternative of the future via thein silicoidentification of plant miRNAs targeting pathogenic bacteria.Graphical Abstract
Publisher
Cold Spring Harbor Laboratory