One year of gluten free diet impacts gut function and microbiome in celiac disease

Abstract

AbstractBACKGROUND & AIMSCurrently, the main treatment option for coeliac disease (CD) is a gluten free diet (GFD). This observational cohort study investigated the impact of CD and 1 year of GFD on gut function and microbiome.METHODS36 newly diagnosed patients and 36 healthy volunteers (HV) were studied at baseline and at 12 months follow up. Small bowel water content (SBWC), whole gut transit time (WGTT) and colon volumes were measured by MRI. Stool samples DNA was subjected to shotgun metagenomic sequencing. Species level abundances and gene functions, including carbohydrate active enzymes (CAZymes) were determined.RESULTSSBWC was significantly higher in people with CD 157±15 mL versus HVs 100±12 mL (p=0.003). WGTT was delayed in people with CD 68±8 hours versus HVs 41±5 hours (p=0.002). The differences reduced after 12 months of GFD but not significantly. Wellbeing in the CD group significantly improved after GFD but did not recover to control values. CD faecal microbiota showed high abundance of proteolytic gene functions, associated withEscherichia coli, EnterobacterandPeptostreptococcus. GFD significantly reducedBifidobacteriaand increasedBlautia wexerelae. Microbiome composition correlated positively with WGTT, colonic volume andAkkermansia municphiliabut negatively withB.wexerelae. Following GFD the reduction in WGTT and colonic volume significantly associated with increased abundance ofB.wexerelae. There were also significant alterations in CAZyme profiles, specifically starch and arabinoxylan degrading families.CONCLUSIONSCD impacted gut function and microbiota. GFD ameliorated but did not reverse these effects, significantly reducingBifidobacteriaassociated with reduced intake of resistant starch and arabinoxylan from wheat.