A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types-Reference-Cited by-同舟云学术

A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types

Published:2024-06-10 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Ben-Simon Yoav^ORCID,Hooper Marcus^ORCID,Narayan Sujatha^ORCID,Daigle Tanya^ORCID,Dwivedi Deepanjali^ORCID,Way Sharon W.^ORCID,Oster Aaron^ORCID,Stafford David A.,Mich John K.^ORCID,Taormina Michael J.^ORCID,Martinez Refugio A.^ORCID,Opitz-Araya Ximena^ORCID,Roth Jada R.^ORCID,Allen Shona^ORCID,Ayala Angela^ORCID,Bakken Trygve E.^ORCID,Barcelli Tyler^ORCID,Barta Stuard^ORCID,Bendrick Jacqueline^ORCID,Bertagnolli Darren^ORCID,Bowlus Jessica^ORCID,Boyer Gabriella^ORCID,Brouner Krissy^ORCID,Casian Brittny^ORCID,Casper Tamara^ORCID,Chakka Anish B.^ORCID,Chakrabarty Rushil^ORCID,Chance Rebecca K.^ORCID,Chavan Sakshi^ORCID,Departee Maxwell^ORCID,Donadio Nicholas^ORCID,Dotson Nadezhda^ORCID,Egdorf Tom^ORCID,Gabitto Mariano^ORCID,Gary Amanda^ORCID,Gasperini Molly^ORCID,Goldy Jeff^ORCID,Gore Bryan B.^ORCID,Graybuck Lucas^ORCID,Greisman Noah^ORCID,Haeseleer Francoise^ORCID,Halterman Carliana^ORCID,Helback Olivia^ORCID,Hockemeyer Dirk^ORCID,Huang Cindy^ORCID,Huff Sydney^ORCID,Hunker Avery^ORCID,Johansen Nelson^ORCID,Juneau Zoe^ORCID,Kalmbach Brian^ORCID,Khem Shannon^ORCID,Kutsal Rana^ORCID,Larsen Rachael^ORCID,Lee Changkyu^ORCID,Lee Angus Y.,Leibly Madison^ORCID,Lenz Garreck H.^ORCID,Liang Elizabeth^ORCID,Lusk Nicholas^ORCID,Malone Jocelin^ORCID,Mollenkopf Tyler^ORCID,Morin Elyse^ORCID,Newman Dakota^ORCID,Ng Lydia^ORCID,Ngo Kiet^ORCID,Omstead Victoria^ORCID,Oyama Alana^ORCID,Pham Trangthanh^ORCID,Pom Christina A.^ORCID,Potekhina Lydia^ORCID,Ransford Shea^ORCID,Rette Dean^ORCID,Rimorin Christine^ORCID,Rocha Dana^ORCID,Ruiz Augustin^ORCID,Sanchez Raymond E.A.^ORCID,Sedeno-Cortes Adriana^ORCID,Sevigny Joshua P.^ORCID,Shapovalova Nadiya^ORCID,Shulga Lyudmila^ORCID,Sigler Ana R.^ORCID,Siverts La’ Akea^ORCID,Somasundaram Saroja^ORCID,Stewart Kaiya^ORCID,Tieu Michael^ORCID,Trader Cameron^ORCID,van Velthoven Cindy T.J.^ORCID,Walker Miranda^ORCID,Weed Natalie^ORCID,Wirthlin Morgan^ORCID,Wood Toren^ORCID,Wynalda Brooke^ORCID,Yao Zizhen^ORCID,Zhou Thomas^ORCID,Ariza Jeanelle^ORCID,Dee Nick^ORCID,Reding Melissa^ORCID,Ronellenfitch Kara^ORCID,Mufti Shoaib^ORCID,Sunkin Susan M.^ORCID,Smith Kimberly A.^ORCID,Esposito Luke^ORCID,Waters Jack^ORCID,Thyagarajan Bargavi^ORCID,Yao Shenqin^ORCID,Lein Ed S.^ORCID,Zeng Hongkui^ORCID,Levi Boaz P.^ORCID,Ngai John^ORCID,Ting Jonathan^ORCID,Tasic Bosiljka^ORCID

Abstract

AbstractThe mammalian cortex is comprised of cells with different morphological, physiological, and molecular properties that can be classified according to shared properties into cell types. Defining the contribution of each cell type to the computational and cognitive processes that are guided by the cortex is essential for understanding its function in health and disease. We use transcriptomic and epigenomic cortical cell type taxonomies from mice and humans to define marker genes and enhancers, and to build genetic tools for cortical cell types. Here, we present a large toolkit for selective targeting of cortical populations, including mouse transgenic lines and recombinant adeno-associated virus (AAV) vectors containing genomic enhancers. We report evaluation of fifteen new transgenic driver lines and over 680 different enhancer AAVs covering all major subclasses of cortical cells, with many achieving a high degree of specificity, comparable with existing transgenic lines. We find that the transgenic lines based on marker genes can provide exceptional specificity and completeness of cell type labeling, but frequently require generation of a triple-transgenic cross for best usability/specificity. On the other hand, enhancer AAVs are easy to screen and use, and can be easily modified to express diverse cargo, such as recombinases. However, their use depends on many factors, such as viral titer and route of administration. The tools reported here as well as the scaled process of tool creation provide an unprecedented resource that should enable diverse experimental strategies towards understanding mammalian cortex and brain function.

Publisher

Cold Spring Harbor Laboratory

Reference69 articles.

1. Genetic strategies to access activated neurons

2. Genetic Dissection of Neural Circuits: A Decade of Progress

3. Morphological diversity of single neurons in molecularly defined cell types

4. Three groups of interneurons account for nearly 100% of neocortical GABAergic neurons

5. Single-nucleus and single-cell transcriptomes compared in matched cortical cell types

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Evaluating Methods for the Prediction of Cell Type-Specific Enhancers in the Mammalian Cortex;2024-08-21

2. Enhancer AAVs for targeting spinal motor neurons and descending motor pathways in rodents and macaque;2024-07-31

3. An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes;2024-07-22

4. Neocortical long-range inhibition promotes cortical synchrony and sleep;2024-06-23