Author:
Furlanis Elisabetta,Dai Min,Leyva Garcia Brenda,Vergara Josselyn,Pereira Ana,Pelkey Kenneth,Tran Thien,Gorissen Bram L.,Vlachos Anna,Hairston Ariel,Huang Shuhan,Dwivedi Deepanjali,Du Sarah,Wills Sara,McMahon Justin,Lee Anthony T.,Chang Edward F.,Razzaq Taha,Qazi Ahmed,Vargish Geoffrey,Yuan Xiaoqing,Caccavano Adam,Hunt Steven,Chittajallu Ramesh,McLean Nadiya,Hewit Lauren,Paranzino Emily,Rice Haley,Cummins Alex C.,Plotnikova Anya,Mohanty Arya,Tangen Anne Claire,Shin Jung Hoon,Azadi Reza,Eldridge Mark A.G.,Alvarez Veronica A.,Averbeck Bruno B.,Alyahyay Mansour,Reyes Vallejo Tania,Soheib Mohammed,Vattino Lucas G.,MacGregor Cathryn P.,Banks Emmie,Olah Viktor Janos,Naskar Shovan,Hill Sophie,Liebergall Sophie,Badiani Rohan,Hyde Lili,Xu Qing,Allaway Kathryn C.,Goldberg Ethan M.,Nowakowski Tomasz J.,Lee Soohyun,Takesian Anne E.,Ibrahim Leena A.,Iqbal Asim,McBain Chris J.,Dimidschstein Jordane,Fishell Gord,Wang Yating
Abstract
SUMMARYIn recent years, we and others have identified a number of enhancers that, when incorporated into rAAV vectors, can restrict the transgene expression to particular neuronal populations. Yet, viral tools to access and manipulate fine neuronal subtypes are still limited. Here, we performed systematic analysis of single cell genomic data to identify enhancer candidates for each of the cortical interneuron subtypes. We established a set of enhancer-AAV tools that are highly specific for distinct cortical interneuron populations and striatal cholinergic neurons. These enhancers, when used in the context of different effectors, can target (fluorescent proteins), observe activity (GCaMP) and manipulate (opto- or chemo-genetics) specific neuronal subtypes. We also validated our enhancer-AAV tools across species. Thus, we provide the field with a powerful set of tools to study neural circuits and functions and to develop precise and targeted therapy.
Publisher
Cold Spring Harbor Laboratory