Author:
Zimbres Flavia M.,Valenciano Ana Lisa,Merino Emilio F.,Florentin Anat,Holderman Nicole R.,He Guijuan,Gawarecka Katarzyna,Skorupinska-Tudek Karolina,Fernández-Murga Maria L.,Swiezewska Ewa,Wang Xiaofeng,Muralidharan Vasant,Cassera Maria Belen
Abstract
Thecis-polyisoprenoid lipids namely polyprenols, dolichols and their derivatives are linear polymers of several isoprene units. In eukaryotes, polyprenols and dolichols are synthesized as a mixture of four or more homologues of different length with one or two predominant species with sizes varying among organisms. Polyprenols have been hardly detectable in eukaryotic cells under normal conditions with the exception of plants and sporulating yeast. Our metabolomics studies revealed thatcis-polyisoprenoids are more prevalent and diverse in the parasitePlasmodium falciparumthan previously postulated as we uncovered activede novobiosynthesis and substantial levels of accumulation of polyprenols and dolichols of 15 to 19 isoprene units. A distinctive polyprenol and dolichol profile both within the intraerythrocytic asexual cycle and between asexual and gametocyte stages was also observed suggesting thatcis-polyisoprenoid biosynthesis changes throughout parasite’s development. In addition, we confirmed the presence of an activecis-prenyltransferase (PfCPT) and that dolichol biosynthesis occurs via reduction of the polyprenol to dolichol by an active polyprenol reductase (PfPPRD) in the malaria parasite. Isotopic labeling and metabolomic analyses of a conditional mutant of PfCPT or PfPPRD suggest that polyprenols may be able to substitute dolichols in their biological functions when dolichol synthesis is impaired inPlasmodium.
Publisher
Cold Spring Harbor Laboratory