Drastic shift in flowering phenology, an instant reproductive isolation mechanism, explains the population structure of Imperata cylindrica in Japan

Abstract

AbstractReproductive isolation plays an important role in population differentiation and speciation, thus enhancing biodiversity in wild plants. Hybridisation sometimes involves rapid reproductive isolation between parents and their hybrids through the novel traits of hybrids derived from a new combination of genomes. Here, we report how a hybrids’ new phenotype contributes to rapid reproductive isolation between two ecotypes of Imperata cylindrica. The two ecotypes differ in their flowering phenology and habitats. An analysis with genetic markers revealed that hybrid populations consisted of only F1 individuals. Both parental ecotypes flowered in spring, but F1s flowered in fall. This drastic shift in flowering phenology prevented backcrossing parental ecotypes to F1. F1s flowered in fall and dispersed seeds in winter. The germination percentage of seeds set on F1 was extremely low in their habitats, and seedlings did not survive due to the low temperatures in winter, resulting in the absence of a F2 generation. In conclusion, flowering phenology mismatch promotes reproductive isolation between parents and F1, resulting in a hybrid population consisting of only F1s.