Abstract
INTRODUCTIONThis protocol describes an alternative method for breeding Astyanax mexicanus, using in vitro fertilization. Sperm collected from the male and eggs collected from the female are placed in a Petri dish, and sperm are activated by the addition of fresh system water. The eggs are observed under low magnification for signs of fertilization, usually marked by the onset of cleavage.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
8 articles.
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