Abstract
INTRODUCTIONThis assay is used for protein quantitation. It is relatively accurate for most proteins, except for small basic polypeptides such as ribonuclease or lysozyme. It is also hampered by detergent concentrations over ~0.2%.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Reference2 articles.
1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
2. Spector D.L., Goldman R.D., and Leinwand L.A. 1998. Determination of protein concentration. In Cells: A laboratory manual, Vols. 1-3, pp. 56.2-56.3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
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