Preparation of Rough Microsomes from Rat Liver

Author:

Sabatini David D.

Abstract

This protocol describes how to prepare rat liver rough microsomes that contain undegraded membrane-bound polysomes and can function very well in an in vitro translation system. It uses endogenous ribonuclease inhibitor in all steps, avoiding pelleting rough microsomes in all steps and sacrificing good recovery.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Reference13 articles.

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4. Depierre J , Dallner G . 1976. Isolation, sub-fractionation and characterization of the endoplasmic reticulum. In Biochemical analysis of membranes (ed. Maddy AH ), pp. 79–131. John Wiley, New York.

5. Feldman RA , Gaetani S , Morimoto T . 1982. Preparation of functional rough microsomes from WI-38 human diploid fibroblasts, HeLa cells and rat liver. In Cancer cell organelles (ed. Reid E , ), pp. 263–292. John Wiley, New York.

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