Towards bone-remodeling-on-a-chip: self-assembling 3D osteoblast-osteoclast coculture in a microfluidic chip

Author:

Vis M.A.M.ORCID,Zhao F.ORCID,Bodelier E.S.R.,Bood C.M.,Bulsink J.,van Doeselaar M.,Amirabadi H. EslamiORCID,Ito K.,Hofmann S.ORCID

Abstract

AbstractHealthy bone is maintained by the process of bone remodeling. An unbalance in this process can lead to pathologies such as osteoporosis which are often studied with animal models. However, data from animals have limited power in predicting the results that will be obtained in human clinical trials. In search for alternatives to animal models, humanin vitromodels are emerging as they address the principle of reduction, refinement, and replacement of animal experiments (3Rs). At the moment, no completein vitromodel for bone-remodeling exists. Microfluidic chips offer great possibilities, particularly because of the dynamic culture options, which are crucial forin vitrobone formation. In this study, a scaffold free, fully human, 3D microfluidic coculture model of bone remodeling is presented. A bone-on-a-chip coculture system was developed in which human mesenchymal stromal cells differentiated into osteoblasts and self-assembled into scaffold free bone-like tissues with the shape and dimensions of human trabeculae. Human monocytes were able to attach to these tissues and to fuse into multinucleated osteoclast-like cells, establishing the coculture. Furthermore, a set-up was developed allowing for long-term (35 days) on-chip cell culture with benefits including continuous fluid-flow, low bubble formation risk, easy culture medium exchange inside the incubator and live cell imaging options. This on-chip coculture is a crucial advance towards developingin vitrobone remodeling models to facilitate drug testing.

Publisher

Cold Spring Harbor Laboratory

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