Telomemore enables single-cell analysis of cell cycle and chromatin condensation

Abstract

AbstractIt is now easy to perform multiome single-cell analysis, including both RNA and ATAC readouts from the same cell. This enables a closer linkage between the two types of modalities, but it remains an open question what more information can be extracted from this type of data. ATAC-seq is normally only used to assay transcription factor binding to open regions. By reanalyzing several large datasets, and generating an atlas of B cells, we show that telomere accessibility can better pinpoint processes related to cell cycle and chromatin condensation. We provide Telomemore, a tool that can extract telomeric reads, and give examples of new findings it enables. Our new findings will aid in the annotation and analysis of single-cell ATAC or multiome datasets.