Abstract
AbstractAdoptive cell therapy (ACT) requires the in vitro expansion of T cells, a process where currently several variables are poorly controlled. As the state and quality of the cells affects the treatment outcome, the lack of insight is problematic. To get a better understanding of the production process and its degrees of freedom, we have generated a multiome CD4 T cell single-cell atlas. We find in particular a JUNB+ epigenetic state, orthogonal to traditional CD4 T cell subtype categorization. This new state is present but overlooked in previous transcriptomic CD4 T cell atlases. We characterize it to be highly proliferative, having condensed and actively remodeled chromatin, and correlating with exhaustion. JUNB+ subsets are also linked to memory formation, as well as circadian rhythm, connecting several important processes into one state. To dissect JUNB regulation, we also derived a gene regulatory network (GRN) and developed a new explainable machine learning package, Nando. We propose potential upstream drivers of JUNB, verified by other atlases and orthogonal data. We expect our results to be relevant for optimizing in vitro ACT conditions as well as modulation of gene expression through novel gene editing.
Publisher
Cold Spring Harbor Laboratory