Alginate-regulating genes are identified in the clinical cystic fibrosis isolate ofPseudomonas aeruginosaPA2192

Abstract

ABSTRACTCystic fibrosis (CF) is a genetic disorder that leads to a buildup of mucus in the lungs ideal for bacterial colonization. WhenPseudomonas aeruginosaenters the CF lung, it undergoes a conversion from nonmucoid to mucoid; colonization by a mucoid strain ofP. aeruginosagreatly increases mortality. The mucoid phenotype is due to the production of alginate. The regulator of alginate production is the AlgT/U sigma factor. The observed phenotypic conversion is due to a mutation in themucAgene coding for an anti-sigma factor, MucA, which sequesters AlgT/U. This mucoid phenotype is unstable when the strains are removed from the lung as they acquire second-site mutations. Thisin vitroreversion phenomenon is utilized to identify novel genes regulating alginate production. Previously, second-site mutations were mapped toalgT/U, algO,andmucP, demonstrating their role in alginate regulation. Most of these studies were performed using a non-CF isolate. It was hypothesized that second site mutations in a clinical strain would be mapped to the same genes. In this study, a clinical, hyper-mucoidP. aeruginosastrain PA2192 was used to study the reversion phenomenon. This study found that PA2192 has a novelmucAmutation which was named themmucA180allele. Twelve colonies were sub-cultured for two weeks without aeration at room temperature in order to obtain nonmucoidsuppressors ofalginateproduction(sap). Only 41sapmutants were stable for more than 48 hours — a reversion frequency of 3.9% as compared to ~90% in laboratory strains showing that PA2192 has a stable mucoid phenotype. This phenotype was restored in 28 of the 41sapmutants when complemented with plasmids harboringalgT/U. Four of thesapmutants are complemented byalgO. Sequence analyses of thealgT/Umutants have found no mutations in the coding region or promoter leading to the hypothesis that there is another, as yet unidentified mechanism of alginate regulation in this clinical strain.