Author:
Digel Leonid,Justesen Mads L.,Bonné Robin,Fransaert Nico,Wouters Koen,Jensen Pia B.,Plum-Jensen Lea E.,Marshall Ian P. G.,Nicolas-Asselineau Louison,Drace Taner,Bøggild Andreas,Hansen John L.,Schramm Andreas,Bøjesen Espen D.,Nielsen Lars P.,Manca Jean V.,Boesen Thomas
Abstract
AbstractCable bacteria encompass at least two genera, and they are known to vary greatly in habitat preferences and filament thickness. We systematically investigated variations and similarities in cellular structures and electrical properties of different cable bacteria strains. Using SEM, TEM, STEM-EDX and ToF-SIMS, we characterized shared features of cable bacteria, such as inner and outer membranes, surface layer and cell junction architecture, as well as strain specific features, like the number and size of periplasmic conductive fibers (PCFs). Our data indicates that the PCFs are organized as loose stranded rope-like structures. With spatially resolved elemental analysis we detected nickel-containing co-factors within the PCF of cable bacteria strains in both genera suggesting a conserved conduction mechanism. Electrical conductivity of different cable bacteria strains showed a range of values covering three orders of magnitude indicating an unknown metabolic adaptation. Using cryogenic electron tomography we discovered multiple polar chemosensory arrays, abundant cytoplasmic inner membrane-attached vesicles (IMVs), polysomes and inner membrane invaginations that shed light on cable bacteria metabolism including complex motility control mechanisms, localized protein synthesis, and membrane remodeling. We propose that the IMVs discovered in this work are novel metabolic hubs closely connected to the unique conductive fiber structure of cable bacteria.
Publisher
Cold Spring Harbor Laboratory
Reference54 articles.
1. How do cyanobacteria glide ?;Microbiology today,2001
2. Motility of Electric Cable Bacteria
3. Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows
4. Trimmomatic: a flexible trimmer for Illumina sequence data
5. Intrinsic electrical properties of cable bacteria reveal an Arrhenius temperature dependence;Scientific Reports. Nature Publishing Group UK,2020
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献