Croton caudatus Geiseler Induces Differentiation of the Acute Myeloid Leukemia cell lines

Abstract

AbstractCroton caudatus Geiseler (CCG) is a traditional medicinal plant and it has also been reported as having anticancer activity. However, most of the studies of the anticancer activity of CGG based only on cytotoxicity and antioxidant properties. The present study investigates the anticancer activity of leaf crude extract of CCG on an acute myeloid leukemia cell line, HL60. The results showed that CCG promotes the monocyte macrophage-like morphology of HL60 cells but it does not cause cytotoxicity on HL60.The results also showed that CCG increased the expression of a marker for monocyte/macrophage differentiation, CD11b, and induced arrest of cell cycle at S phase. Further, the results indicate that leaf the crude extract of CCG has a very high ROS activity. The present study is the first report of CCG inducing cell differentiation. The overall results suggest that CCG is a promising agent for differentiation induction chemotherapy (DIC) for leukemia.