Author:
Fonseca Cindy L.,Malaby Heidi L.H.,Sepaniac Leslie A.,Martin Whitney,Byers Candice,Czechanski Anne,Messinger Dana,Tang Mary,Ohi Ryoma,Reinholdt Laura G.,Stumpff Jason
Abstract
SUMMARYChromosome alignment at the equator of the mitotic spindle is a highly conserved step during cell division, however, its importance to genomic stability and cellular fitness are not understood. Normal mammalian somatic cells lacking Kif18A function complete cell division without aligning chromosomes. These alignment-deficient cells display normal chromosome copy numbers in vitro and in vivo, suggesting that chromosome alignment is largely dispensable for maintenance of euploidy. However, we find that loss of chromosome alignment leads to interchromosomal compaction defects during anaphase, abnormal organization of chromosomes into a single nucleus at mitotic exit, and the formation of micronuclei in vitro and in vivo. These defects slow cell proliferation and reduce postnatal growth and survival with variable penetrance in mice. Our studies support a model in which the alignment of mitotic chromosomes promotes proper nuclear envelope reassembly and continued proliferation by ensuring that chromosomes segregate as a compact mass during anaphase.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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