Metabolic specialization drives reduced pathogenicity inPseudomonas aeruginosaCF clinical isolates

Author:

Pedersen Bjarke H.ORCID,Simões Filipa B.ORCID,Pogrebnyakov IvanORCID,Welch Martin,Johansen Helle Krogh,Molin Søren,La Rosa RuggeroORCID

Abstract

AbstractMetabolism provides the foundation for all cellular functions. During persistent infections, in adapted pathogenic bacteria metabolism functions radically differently compared with more naïve strains. Whether this is simply a necessary accommodation to the persistence phenotype or if metabolism plays a direct role in achieving persistence in the host is still unclear. Here, we characterize a convergent shift in metabolic function(s) linked with the persistence phenotype duringPseudomonas aeruginosacolonization in the airways of people with cystic fibrosis. We show that clinically-relevant mutations in the key metabolic enzyme, pyruvate dehydrogenase, lead to a host-specialized metabolism together with a lower virulence and immune response recruitment. These changes in infection phenotype are mediated by impaired type III secretion system activity and by secretion of the antioxidant metabolite, pyruvate, respectively. Our results show how metabolic adaptations directly impinge on persistence and pathogenicity in this organism.

Publisher

Cold Spring Harbor Laboratory

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