Transcription and replication organize cohesin-dependent chromosome loops

Abstract

AbstractGenome function and stability is strictly dependent on regulated folding of chromosomes in space and time. Chromosome loop formation by the protein complex cohesin is a central feature of this multilayer organization1. Accumulating evidence indicates that cohesin creates loops by extruding chromosomal DNA through its ring-like structure in a process that is controlled by the cohesin loading factor Scc2 and the unloader Wpl11–4. Cohesin’s chromosomal positioning is affected by transcription in both yeast and human cells5,6, and the complex localizes in the vicinity of replication forks7. However, if transcription directly influences chromosome looping remains an unresolved question, and the threedimensional organization of replicating chromosomes is unknown. Here we show that transcription and replication machineries create chromosome loop boundaries. We find that drug-induced depletion of chromosome-bound RNA polymerases triggers a rapid expansion of cohesin-dependent chromosome loops in the budding yeast Saccharomyces cerevisiae. New loop boundaries also form at a few highly expressed genes induced by the cellular stress response caused by the drug. The results also reveal that S-phase chromosomes are shaped by cohesin-dependent loops organized by transcription, with additional loop anchors at replication forks. Together, our results show that replication and transcription control the three-dimensional organization of the genome by blocking the progression of loop-forming cohesin. They also open for the possibility that the resulting positioning of loop-forming cohesin in the vicinity of transcription and replication machineries is part of cohesin’s functions in transcription control, sister chromatid cohesion and maintenance of fork stability.