Acetic acid is a superior acidifier for sub-nanogram and single cell proteomic studies

Abstract

AbstractA recent study demonstrated a substantial signal increase when employing a 0.5% acetic acid buffer additive instead of the traditional 0.1% formic acid used in shotgun proteomics. In this study I compare these two buffers for a dilution series of tryptic digests down to 20 picograms peptide on column on a TIMSTOF single cell proteome (SCP) system. I observe a comparable relative level of signal increase as previously reported, which translates to improvements in proteome coverage at every peptide load assessed. The relative increase in peptide identifications is more apparent at lower concentrations with a striking 1.8-fold more peptides identified at 20 pg peptide load, resulting in over 2,000 protein groups identified in 30 minutes on this system. These results translate well to isolated single human cancer cells allowing over 1,000 protein groups to be identified in single human cells processed using a simple one step method in standard 96-well plates.All vendor raw and processed data has been made publicly available atwww.massive.ucsd.eduand can be accessed as MSV000092563.Abstract graphic