Abstract
AbstractRATIONALEPseudomonas aeruginosa(P.a.) is the major bacterial pathogen colonizing the airways of adult cystic fibrosis (CF) patients and causes chronic infections that persist despite antibiotic therapy. Intracellular bacteria may represent an unrecognized reservoir of bacteria that evades the immune system and antibiotic therapy. While the ability ofP.a. to invade and survive within epithelial cells has been describedin vitroin different epithelial cell models, evidence of this intracellular lifestyle in human lung tissues is currently lacking.OBJECTIVESTo detect and characterize intracellularP.a. in CF airway epithelium from human lung explant tissues.METHODSWe sampled the lung explant tissues from CF and non-CF patients undergoing lung transplantation and analyzed lung tissue sections for the presence of intracellularP.a. by quantitative culture and microscopy, in parallel to histopathology and airway morphometry.MEASUREMENTS AND MAIN RESULTSP.a. was isolated from the lungs of 7 CF patients undergoing lung transplantation. Microscopic assessment revealed the presence of intracellularP.a. within airway epithelial cells in 3 out of the 7 lungs analyzed, at a varying but rare frequency. We observed those events occurring in lung regions with high bacterial burden.CONCLUSIONThis is the first study describing the presence of intracellularP.a. in CF lung tissues. While intracellularP.a. in airway epithelial cells are likely relatively rare events, our findings highlight the plausible occurrence of this intracellular bacterial reservoir in chronic CF infections.
Publisher
Cold Spring Harbor Laboratory