Author:
Faget Julien,Boivin Gael,Ancey Pierre-Benoit,Gkasti Aspasia,Mussard Julie,Engblom Camilla,Pfirschke Christina,Vazquez Jessica,Bendriss-Vermare Nathalie,Caux Christophe,Vozenin Marie-Catherine,Pittet Mikael J.,Gunzer Matthias,Meylan Etienne
Abstract
SummaryNeutrophils orchestrate the innate immune response against microorganisms and are increasingly recognized to modulate cancer development in primary tumors and metastases. To address their functionin vivo, different approaches are used, the most common ones relying on antibody-mediated neutrophil depletion. By comparing the effects of two widely used antibodies, we demonstrate a strong efficacy but a lack of specificity for anti-Gr1. In contrast, anti-Ly6G lacks neutrophil-depletion capacity in C57BL/6 mice, which can be explained by an insufficient celerity of neutrophil clearance that is counterbalanced by exacerbated mobilization of immature cells. When combined with a secondary antibody, anti-Ly6G treatment results in specific and efficient neutrophil depletion. Using a mouse model of lung adenocarcinoma, we demonstrate the efficacy of this new approach to diminish primary tumor growth and propose the existence of a local intercellular communication between neutrophils and alveolar macrophages that fosters regulatory T cell proliferation in lung cancer.
Publisher
Cold Spring Harbor Laboratory
Cited by
17 articles.
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