Identification of radiation responsive proteins from Spleen and Intestine tissue of mice using Mass Spectrometry approach

Abstract

AbstractPurposeExposure to ionizing radiation (IR) can cause tissue damage, which is difficult to diagnose and treat as no biomarker is available for detection. We aimed to identify proteomic signature of radiation exposure (9.5Gry) in mice and to assess the utility of Podophyllotoxin extract (PTOX) in preventing radiation injury.Materials and MethodsSpleen and Small intestinal (SI) tissues were taken from control and lethally irradiated mice at different time intervals with or without pre-treatment with Podophyllotoxin extract. Proteins were identified using Mass Spectrometry and matched with Peptide Mass Fingerprinting.ResultsWe found multiple differentially expressed radiation responsive proteins from Spleen and SI tissues in irradiated mice at 24 hours and 30 days in comparison to healthy controls (p<0.05). Differentially expressed proteins like Chromosome transmission fidelity factor ano thath 18 homolog (CTF18) and Rho GTPase-activating protein from spleen and Acta_Mouse protein from SI were identified. These proteins disappeared at 48 hrs. after IR, but re-appeared after 13 days and fully recovered at 30 days in Podophyllotoxin treated group.ConclusionsSuch proteins may be useful in early detection of radiation exposure. Pre-treatment with Podophyllotoxin leads to recovery of the disappeared proteins and improved survival following exposure to irradiation.