Author:
Machold Robert,Dellal Shlomo,Valero Manuel,Zurita Hector,Kruglikov Illya,Meng John,Hanson Jessica L.,Hashikawa Yoshiko,Schuman Benjamin,Buzsáki György,Rudy Bernardo
Abstract
SummaryCortical GABAergic interneurons (INs) represent a diverse population of mainly locally projecting cells that provide specialized forms of inhibition to pyramidal neurons and other INs. Most recent work on INs has focused on subtypes distinguished by expression of Parvalbumin (PV), Somatostatin (SST), or Vasoactive Intestinal Peptide (VIP). However, a fourth group that includes neurogliaform cells (NGFCs) has remained enigmatic due to a lack of genetic tools. Here, we show that these INs can be accessed experimentally using intersectional genetics with the geneId2. We find that outside of layer 1 (L1), the majority of Id2 INs are NGFCs that express high levels of neuropeptide Y (NPY) and exhibit a late-spiking firing pattern, with extensive local connectivity. While much sparser, non-NGFC Id2 INs had more variable properties, with most cells corresponding to a diverse group of INs that strongly expresses the neuropeptide CCK.In vivo, using silicon probe recordings, we observed several distinguishing aspects of NGFC activity, including a strong rebound in activity immediately following the cortical down state during NREM sleep. Our study provides insights into IN diversity and NGFC distribution and properties, and outlines an intersectional genetics approach for further study of this neglected group of INs.
Publisher
Cold Spring Harbor Laboratory
Cited by
2 articles.
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