Author:
Haussmann Irmgard U.,Dix Thomas C.,Dezi Veronica,Hans Abdullah I.,Arnold Roland,Soller Matthias
Abstract
SUMMARYSingle guide RNA (sgRNA) directs Cas9 nuclease for gene-specific scission of double-stranded DNA. High Cas9 activity is essential for efficient gene editing to generate gene deletions and gene replacements by homologous recombination. Here we describe constraints in sgRNA design originating from maintaining the secondary structure of the sgRNA that is essential for high- efficiency DNA scission of Cas9, but aberrant with about 50% of sequences adjacent to PAM sites inDrosophila. We developed an sgRNA design tool (PlatinumCRISPR) to evaluate base-pairing for optimal design of highly efficient sgRNAs for Cas9 genome editing. Applied to generate gene deletions inDrosophila Ythdc1andYthdf, that bind toN6methylated adenosines (m6A) in mRNA we further discovered, that generating small deletions with sgRNAs and Cas9 leads to ectopic reinsertion of the deleted DNA fragment elsewhere in the genome. These insertions, however, can be removed by standard genetic recombination and chromosome exchange.
Publisher
Cold Spring Harbor Laboratory