Genomic evaluation of a case ofLacticaseibacillus rhamnosusendocarditis: comparison with commercial yogurt isolates and examination of global population structure

Abstract

ABSTRACTProbiotics, including yogurt commonly containing bacteria of theLactobacillusgroup, are widely used to promote microbiome health and to prevent gastrointestinal or other infections, especially in the setting of antibiotic use. Rarely, however,Lactobacillusbacteria have caused invasive or severe infections in patients. In this report, we present a patient with fatalLacticaseibacillusbacteremia and endocarditis who frequently consumed yogurt. Whole genome sequencing identified the patient’s bloodstream isolate asLacticaseibacillus(formerlyLactobacillus)rhamnosus, and sequencing ofLactobacillusisolates from selected commercial yogurt preparations revealed one isolate nearly identical to the patient’s isolate, differing by only one nucleotide (adenine to guanine substitution leading to a M1A amino acid change in the start codon of thenrdRgene). To place these sequences into broader context ofL. rhamnosusstrains, we compared them to 602L. rhamnosusgenome sequences publicly available through the National Center for Biotechnology Information repository. Both isolates belonged to a clade, identified in this report as clade YC, composed of mostly gastrointestinal isolates from healthy individuals, some of which also differed by only a single nucleotide from the patient’s isolate. Members of clade YC had only rarely been previously identified in invasive infections. In summary, theLacticaseibacillusstrain causing this patient’s infection may have originated from a commercial dairy product or from the commensal flora of his gastrointestinal tract. This study demonstrates that whole genome sequencing may be insufficient to reliably determine the source of invasive infections caused byL. rhamnosus.IMPORTANCEActive culture and probiotic foods and supplements are often used to promote gut health. Though largely considered safe for human consumption, there are rare reports of severe infections linked to bacterial species used in these products. Using whole genome sequencing, we examined a case of fatal infection withLacticaseibacillus rhamnosusin the patient’s blood. By comparing the bacterial genome sequence from the patient to strains cultured from 4 commercial yogurt samples and 602 publicly available sequences ofL. rhamnosusfrom around the world, we found that it was nearly identical to one of the yogurt strains and equally similar to other strains from both probiotics and healthy individuals’ gastrointestinal tracts. These findings show that even with whole genome sequencing it can be difficult to determine the source of an invasiveL. rhamnosusinfection, whether from recent consumption of a probiotic or dairy product versus from the gastrointestinal microbiome.