Author:
Sokal Aurélien,Barba-Spaeth Giovanna,Fernández Ignacio,Broketa Matteo,Azzaoui Imane,La Selle Andrea de,Vandenberghe Alexis,Fourati Slim,Roeser Anais,Meola Annalisa,Bouvier-Alias Magali,Crickx Etienne,Languille Laetitia,Michel Marc,Godeau Bertrand,Gallien Sébastien,Melica Giovanna,Nguyen Yann,Zarrouk Virginie,Canoui-Poitrine Florence,Noizat-Pirenne France,Megret Jérôme,Pawlotsky Jean-Michel,Fillatreau Simon,Bruhns Pierre,Rey Felix A.,Weill Jean-Claude,Reynaud Claude-Agnès,Chappert Pascal,Mahévas Matthieu
Abstract
SummaryHow a previous SARS-CoV-2 infection may amplify and model the memory B cell (MBC) response elicited by mRNA vaccines was addressed by a comparative longitudinal study of two cohorts, naive individuals and disease-recovered patients, up to 2 months after vaccination. The quality of the memory response was assessed by analysis of the VDJ repertoire, affinity and neutralization against variants of concerns (VOC), using unbiased cultures of 2452 MBCs. Upon boost, the MBC pool of recovered patients selectively expanded, further matured and harbored potent neutralizers against VOC. Maturation of the MBC response in naive individuals was much less pronounced. Nevertheless, and as opposed to their weaker neutralizing serum response, half of their RBD-specific MBCs displayed high affinity towards multiple VOC and one-third retained neutralizing potency against B.1.351. Thus, repeated vaccine challenges could reduce these differences by recall of affinity-matured MBCs and allow naive vaccinees to cope efficiently with VOC.
Publisher
Cold Spring Harbor Laboratory
Cited by
5 articles.
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