In vitro Isolation of Treponema pallidum subsp. pallidum from Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions-Reference-Cited by-同舟云学术

In vitro Isolation of Treponema pallidum subsp. pallidum from Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions

Published:2022-09-14 Issue: Volume: Page:
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Author:

Tantalo Lauren C.,Molini Barbara J.,Bose Mahashweta,Klausner Jeffrey D.,Giacani Lorenzo^ORCID

Abstract

ABSTRACTIsolation of Treponema pallidum subsp. pallidum strains still relies on rabbit intratesticular inoculation of clinical samples. Here, we report an alternative isolation approach based on the inoculation of fresh and frozen needle aspirates of primary experimental lesions into culture plates suitable for in vitro propagation of the syphilis agent.SUMMARYWe report on a rabbit-free in vitro isolation approach for syphilis strains that can be easily adapted to clinical specimens.

Publisher

Cold Spring Harbor Laboratory

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