CircPLXNA2affects the proliferation and apoptosis of myoblast throughcircPLXNA2/gga-miR-12207-5P/MDM4axis

Abstract

AbstractBackgroundcircRNAs are new-identified special endogenous RNA molecules that covalently close a loop by backsplicing with pre-mRNA.In the cytoplasm, circRNAs would act as molecular sponges to bind with specific miRNA to promote the expression of target genes. However, there is still in its fancy of knowing circRNA functional alternation in skeletal myogenesis. In this study, we favor a model to identify the circRNA-miRNA-mRNA interaction network in which the axis may be implicated in the progression of chicken primary myoblasts (CPMs) myogenesis by a combination of multi-omics (i.e., circRNA-seq and ribo-seq).ResultsIn total, 314 circRNA-miRNA-mRNA regulatory axis containing 66 circRNAs, 70 miRNAs, and 24 mRNAs that may be relevant to myogenesis were collected. With these, thecircPLXNA2-gga-miR-12207-5P-MDM4axis aroused our research interest. ThecircPLXNA2is highly differentially expressed during differentiation versus proliferation. It was demonstrated thatcircPLXNA2inhibited the process of apoptosis while at the same time stimulating cell proliferation.Furthermore, we demonstrated thatcircPLXNA2could inhibit the repression ofgga-miR-12207-5ptoMDM4by directing binding togga-miR-12207-5p, thereby restoringMDM4expression.ConclusionsCircPLXNA2could function as a competing endogenous RNA (ceRNA) to inhibit the repression ofgga-miR-12207-5ptoMDM4by directing binding togga-miR-12207-5p, thereby recovering the expression ofMDM4.