Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology
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Published:2024-01-04
Issue:1
Volume:25
Page:680
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Diallo Leïla Halidou1, Mariette Jérôme2, Laugero Nathalie1, Touriol Christian3ORCID, Morfoisse Florent1ORCID, Prats Anne-Catherine1ORCID, Garmy-Susini Barbara1ORCID, Lacazette Eric1ORCID
Affiliation:
1. U1297-I2MC, INSERM, University of Toulouse, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse, France 2. MIAT, University of Toulouse, INRAE, 31326 Castanet-Tolosan, France 3. UMR1037 INSERM, University of Toulouse, 2 Avenue Hubert Curien, 31100 Toulouse, France
Abstract
Circular RNAs (circRNAs) are a recently characterized family of gene transcripts forming a covalently closed loop of single-stranded RNA. The extent of their potential for fine-tuning gene expression is still being discovered. Several studies have implicated certain circular RNAs in pathophysiological processes within vascular endothelial cells and cancer cells independently. However, to date, no comparative study of circular RNA expression in different types of endothelial cells has been performed and analysed through the lens of their central role in vascular physiology and pathology. In this work, we analysed publicly available and original RNA sequencing datasets from arterial, veinous, and lymphatic endothelial cells to identify common and distinct circRNA expression profiles. We identified 4713 distinct circRNAs in the compared endothelial cell types, 95% of which originated from exons. Interestingly, the results show that the expression profile of circular RNAs is much more specific to each cell type than linear RNAs, and therefore appears to be more suitable for distinguishing between them. As a result, we have discovered a specific circRNA signature for each given endothelial cell type. Furthermore, we identified a specific endothelial cell circRNA signature that is composed four circRNAs: circCARD6, circPLXNA2, circCASC15 and circEPHB4. These circular RNAs are produced by genes that are related to endothelial cell migration pathways and cancer progression. More detailed studies of their functions could lead to a better understanding of the mechanisms involved in physiological and pathological (lymph)angiogenesis and might open new ways to tackle tumour spread through the vascular system.
Funder
Ligue Contre le Cancer 31, Institut National de la Santé et de la Recherche Médicale
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference100 articles.
1. Holzapfel, G.A., and Ogden, R.W. (2003). Biomechanics of Soft Tissue in Cardiovascular Systems, Springer. International Centre for Mechanical Sciences. 2. Pittman, R.N. (2011). The Circulatory System and Oxygen Transport, Morgan & Claypool Life Sciences. 3. The Physiology of the Lymphatic System;Swartz;Adv. Drug Deliv. Rev.,2001 4. Krüger-Genge, A., Blocki, A., Franke, R.-P., and Jung, F. (2019). Vascular Endothelial Cell Biology: An Update. Int. J. Mol. Sci., 20. 5. Lymphangiogenesis in Development and Human Disease;Alitalo;Nature,2005
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