Different Expressions of IL-1 TNF-α P-selectin mRNA by Endothelial Cells after Vein Thrombosis

Abstract

[Abstract]ObjectiveExperiments were designed to compare the expressions of IL-1 TNF-α P-selectin mRNA by porcine endothelial cells after vein thrombosis.MethodsIVC under the renal vein of 20 porcines were ligated to induce thrombosis modes. These thrombosed veins were divided into three groups:group A-one day after thrombosis, group B-four days after thrombosis and group C-seven days after thrombosis. The other pigs were given the shame operation as a contro group (group D). The mRNA levels of IL-1、 TNF-α and P-selectin expressed by porcine endothelial cells in three groups were analy sed by semi quantitative RT-PCR. Endothelial cells were harvested with collagenase II.ResultsThe purity of endothelial cells harvested was 99.42 ±0.07. The expression of IL-1 was detained only in group A while TNF-αreached its peak in group B(P<0.05) and P-selectin increased gradually with the days passing by(P<0.05).ConclusionEndothelial cells are not only the target cells of inflammatory mediators, but also can express a variety of active factors to promote venous thrombosis. Expression of TNF-α mRNA is increased gradually in the early period of vein thrombosis whileP-selectin in the acute period; IL-1 mRNA was transiently expressed only in the early stage of thrombosis.