Characterization of Acid Soluble Collagen from Redbelly Yellowtail Fusilier Fish Skin (Caesio cuning)

Abstract

<p>Fish skin can be used as raw material for producing collagen. The collagen can be extracted by chemical<br />or combination of chemical and enzymatic processes. Extraction of collagen chemically can do with the<br />acid process that produces acid soluble collagen (ASC). This study aimed to determine the optimum<br />concentration and time of pretreatment and extraction, also to determine the characteristics of the acid<br />soluble collagen from the skin of yellow tail fish. Extraction of collagen done by pretreatment using NaOH at<br />the concentration of 0.05; 0.1; and 0.15 M and extraction using acetic acid at the concentration of 0.3; 0.5; and<br />0.7 M. Pretreatment NaOH with concentration 0.05 M and soaking time of 8 hours is the best combination<br />for eliminating non collagen protein. Combination treatment of acetic acid at the concentration of 0.3 M<br />for 3 days obtained the best solubility. The yield of collagen ASC was 18.4±1.49% (db) and 5.79±0.47%<br />(wb). Amino acid composition that is dominant in the ASC collagen was glycine (25.09±0.003%), alanine<br />(13.71±0.075%), and proline (12.15±0.132%). Collagen from yellow tail fish skin has α1, α2, β and γ<br />protein structure with the molecular weight of 125, 113, 170-181, and 208 KDa. The transition and melting<br />temperatures of collagen were 67.69oC and 144.4oC. The surface structure of collagen by analysis of SEM has<br />fibers on the surface.<br />Keywords: cholesterol, fatty acids, meat tissue, proximate, red snapper (L. argentimaculatus)</p>